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E2F8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

E2F8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G109829-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human E2F8 (NM_024680), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
E2F family member 8 | E2F-8 | E2F8 | E2F transcription factor 8 | Transcription factor E2F8
Origin Species
Human
NCBI GenBank
NM_024680
SwissProt
A0AVK6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENEKENLFC EPHKRGLMKT PLKESTTANI VLAEIQPDFG PLTTPTKPKE GSQGEPWTPT
61ANLKMLISAV SPEIRNRDQK RGLFDNRSGL PEAKDCIHEH LSGDEFEKSQ PSRKEKSLGL
121LCHKFLARYP NYPNPAVNND ICLDEVAEEL NVERRRIYDI VNVLESLHMV SRLAKNRYTW
181HGRHNLNKTL GTLKSIGEEN KYAEQIMMIK KKEYEQEFDF IKSYSIEDHI IKSNTGPNGH
241PDMCFVELPG VEFRAASVNS RKDKSLRVMS QKFVMLFLVS TPQIVSLEVA AKILIGEDHV
301EDLDKSKFKT KIRRLYDIAN VLSSLDLIKK VHVTEERGRK PAFKWTGPEI SPNTSGSSPV
361IHFTPSDLEV RRSSKENCAK NLFSTRGKPN FTRHPSLIKL VKSIESDRRK INSAPSSPIK
421TNKAESSQNS APFPSKMAQL AAICKMQLEE QSSESRQKVK VQLARSGPCK PVAPLDPPVN
481AEMELTAPSL IQPLGMVPLI PSPLSSAVPL ILPQAPSGPS YAIYLQPTQA HQSVTPPQGL
541SPTVCTTHSS KATGSKDSTD ATTEKAANDT SKASASTRPG SLLPAPERQG AKSRTREPAG
601ERGSKRASML EDSGSKKKFK EDLKGLENVS ATLFPSGYLI PLTQCSSLGA ESILSGKENS
661SALSPNHRIY SSPIAGVIPV TSSELTAVNF PSFHVTPLKL MVSPTSVAAV PVGNSPALAS
721SHPVPIQNPS SAIVNFTLQH LGLISPNVQL SASPGSGIVP VSPRIESVNV APENAGTQQG
781RATNYDSPVP GQSQPNGQSV AVTGAQQPVP VTPKGSQLVA ESFFRTPGGP TKPTSSSCMD
841FEGANKTSLG TLFVPQRKLE VSTEDVH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

867 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.