LSBio
E. coli ackA Protein (Recombinant His)

E. coli ackA Protein (Recombinant His)

ProteinLSBio Guarantee
Expression SystemEscherichia coli (Prokaryotic)
Format:Liquid
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$267.00each
Catalog Number: LS-G3056-20

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Overview

Description
ackA catalyzes the formation of acetyl phosphate from acetate and ATP. This protein can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. Recombinant E.coli ackA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Category
Proteins & Peptides > Proteins > Recombinant Proteins
Origin Species
E. coli
NCBI GenBank
NP_416799.1

Protein

Expression System
Escherichia coli (Prokaryotic)
Fusion Tag
Hexahistidine Tag
Protein Type
Recombinant
Amino Acid Sequence
11-400aa|MG SSHHHHHHSS GLVPRGSHMG SMSSKLVLVL NCGSSSLKFA IIDAVNGEEY
61LSGLAECFHL PEARIKWKMD GNKQEAALGA GAAHSEALNF IVNTILAQKP ELSAQLTAIG
121HRIVHGGEKY TSSVVIDESV IQGIKDAASF APLHNPAHLI GIEEALKSFP QLKDKNVAVF
181DTAFHQTMPE ESYLYALPYN LYKEHGIRRY GAHGTSHFYV TQEAAKMLNK PVEELNIITC
241HLGNGGSVSA IRNGKCVDTS MGLTPLEGLV MGTRSGDIDP AIIFHLHDTL GMSVDAINKL
301LTKESGLLGL TEVTSDCRYV EDNYATKEDA KRAMDVYCHR LAKYIGAYTA LMDGRLDAVV
361FTGGIGENAA MVRELSLGKL GVLGFEVDHE RNLAARFGKS GFINKEGTRP AVVIPTNEEL
421VIAQDASRLT A

431 aa

Format

Purity
>95%
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
20 mM Tris-HCl, pH 8.0, 0.15 M NaCl, 1 mM DTT, 20% Glycerol
Purity Measurement Method
SDS-PAGE (Coomassie Stain)

Storage & Handling

Storage Conditions
Short term: store at 2°C to 8°C for up to 1 week. Long term: aliquot and store at -20°C to -80°C. Avoid freeze-thaw cycles.

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