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DZIP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DZIP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113803-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DZIP3 (NM_014648), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DAZ-interacting protein 3 | HRUL138 | KIAA0675 | UURF2 | PPP1R66 | DZIP3 | UURF2 ubiquitin ligase
Origin Species
Human
NCBI GenBank
NM_014648
SwissProt
Q86Y13

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
138.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSLPDEFFV RHPAVEDQRK EETENKLEKS SGQLNKQEND IPTDLVPVNL LLEVKKLLNA
61INTLPKGVVP HIKKFLQEDF SFQTMQREVA ANSQNGEEIV PALTLRFLIT QLEAALRNIQ
121AGNYTAHQIN IGYYLTLLFL YGVALTERGK KEDYTEAENK FLVMKMMIQE NEICENFMSL
181VYFGRGLLRC AQKRYNGGLL EFHKSLQEIG DKNDHWFDID PTEDEDLPTT FKDLLNNFIK
241TTESNIMKQT ICSYLDCERS CEADILKNTS YKGFFQLMCS KSCCVYFHKI CWKKFKNLKY
301PGENDQSFSG KKCLKEGCTG DMVRMLQCDV PGIVKILFEV VRKDEYITIE NLGASYRKLI
361SLKITDTDIR PKISLKFNTK DEMPIFKLDY NYFYHLLHII IISGTDIVRQ IFDEAMPPPL
421LKKELLIHKN VLESYYNHLW TNHPLGGSWH LLYPPNKELP QSKQFDLCLL LALIKHLNVF
481PAPKKGWNME PPSSDISKSA DILRLCKYRD ILLSEILMNG LTESQFNSIW KKVSDILLRL
541GMMQEDIDKV KENPIENISL DYHQLSVYLG IPVPEIIQRM LSCYQQGIAL QSITGSQRIE
601IEELQNEEEE LSPPLMEYNI NVKSHPEIQF AEINKDGTSI PSESSTESLK DLQEVKSKQR
661KKKKTKNKKN KDSKEDQVPY VVEKEEQLRK EQANPHSVSR LIKDDASDVQ EDSAMEDKFY
721SLDELHILDM IEQGSAGKVT TDYGETEKER LARQRQLYKL HYQCEDFKRQ LRTVTFRWQE
781NQMQIKKKDK IIASLNQQVA FGINKVSKLQ RQIHAKDNEI KNLKEQLSMK RSQWEMEKHN
841LESTMKTYVS KLNAETSRAL TAEVYFLQCR RDFGLLHLEQ TEKECLNQLA RVTHMAASNL
901ESLQLKAAVD SWNAIVADVR NKIAFLRTQY NEQINKVKQG FALSTLPPVQ LPPPPPSPEI
961LMQQFLGRPL VKESFFRPIL TVPQMPAVCP GVVSATGQPR APLMTGIAWA LPAPVGDAVP
1021PSAGLRSDPS IMNWERITDR LKTAFPQQTR KELTDFLRKL KDAYGKSLSE LTFDEIVCKI
1081SQFIDPKKSQ SQGKSVSNVN CVSPSHSPSQ PDAAQPPKPA WRPLTSQGPA TWEGASNPDE
1141EEEEEEPCVI CHENLSPENL SVLPCAHKFH AQCIRPWLMQ QGTCPTCRLH VLLPEEFPGH
1201PSRQLPKI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1208 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.