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DZANK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DZANK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119075-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DZANK1 (NM_001099407), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKRD64 | Ankyrin repeat domain 64 | BA189K21.1 | BA189K21.8 | C20orf12 | C20orf84 | DJ568F9.2 | DZANK1
Origin Species
Human
NCBI GenBank
NM_018152
SwissProt
Q9NVP4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTAGSVCVPQ IIPLRVPQPG KANHEIDNNT LLEMKSDTPD VNIYYTLDGS KPEFLKRIGY
61GENNTFKYIK PITLPDGKIQ VKAIAVSKDC RQSGIVTKVF HVDYEPPNIV SPEDNVENVL
121KDSSRQEFKN GFVGSKLKKK YKNSENQRSW NVNLRKFPES PLEIPAYGGG SGSRPPTRQS
181QSPGFAHVSG QKCLTSTEIM RIQRETDFLK CAHCLAPRPS DPFARFCQEC GSPVPPIFGC
241RLPPPEGAQM GLCAECRSLV PMNTPICVVC EAPLALQLQP QASLHLKEKV ICRACGTGNP
301AHLRYCVTCE GALPSSQESM CSGDKAPPPP TQKGGTISCY RCGRWNLWEA SFCGWCGAML
361GIPAGCSVCP KCGASNHLSA RFCGSCGICV KSLVKLSLDR SLALAAEEPR PFSESLNIPL
421PRSDVGTKRD IGTQTVGLFY PSGKLLAKKE QELASQKQRQ EKMSDHKPLL TAISPGRGYW
481RRQLDHISAH LRCYAQNNPE FRALIAEPRM GKLISATVHE DGCEVSIRLN YSQVSNKNLY
541LNKAVNFSDH LLSSAAEGDG GLCGSRSSWV SDYSQSTSDT IEKIKRIKNF KTKTFQEKKE
601QLIPENRLLL KEVGPTGEGR VSVIEQLLDE GADPNCCDED NRPVITVAVM NKHHEAIPVL
661VQRGADIDQQ WGPLRNTALH EATLLGLAGR ESTATLLGCN ASIQKKNAGG QTAYDLALNT
721GDDLVTSLFA AKFGQGLEDQ LAQTRSLSLD DC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

752 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.