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DYTN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DYTN Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G123954-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Dystrotelin(DYTN) (NM_001093730), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DYTN | Dystrotelin
Origin Species
Human
SwissProt
A2CJ06

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDPDKQDALN SIENSIYRTA FKLQSVQTLC QLDLIDSSLI QQVLLRPSFW EARKHSLSVQ
61QLSQALQELF QKAREENPGQ VHPRAPELTL SLLTTMYNSK GTGFLQLMPA AAALITLSGD
121SPLSKYRALF QLYAENSRGG YDSGPRMTRR VLRKLLTDLQ QIPTFVGESR ALCPVESATR
181SCFQGVLSPA IKEEKFLSWV QSEPPILLWL PTCHRLSAAE RVTHPARCTL CRTFPITGLR
241YRCLKCLNFD ICQMCFLSGL HSKSHQKSHP VIEHCIQMSA MQNTKLLFRT LRNNLLQGRC
301RKKEAARRQQ LLDQVNPKGV PHHAQARLLK KQLNQYKDKL QAIYTSQEER ICRFETRIHK
361LKTNQDSLWT KLQQIRRDLQ ARLQPPGPSS SSFQNVGNKV DHSSTEKVPK GGDYLQIKNA
421TEDASTGEPL PKLDEVDRSH RSHTNAEHAL RNPESPETTL HSTRAQSQTQ KMPQKVISAL
481PSYQEGLKQD IPKMVPAEMS SPALAAVEKK EAGNIKERKD ELEEEELQEL LSKLMDAFNL
541ETPSGPESSV NMDLYSGAQR VCRAFSALVD QIALPNLK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

578 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.