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DYRK4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DYRK4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G100146-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DYRK4 (NM_003845), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Protein kinase DYRK4 | DYRK4
Origin Species
Human
NCBI GenBank
AF263541
SwissProt
Q9NR20

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MPASELKASE IPFHPSIKTQ DPKAEEKSPK KQKVTLTAAE ALKLFKNQLS PYEQSEILGY
61AELWFLGLEA KKLDTAPEKF SKTSFDDEHG FYLKVLHDHI AYRYEVLETI GKGSFGQVAK
121CLDHKNNELV ALKIIRNKKR FHQQALMELK ILEALRKKDK DNTYNVVHMK DFFYFRNHFC
181ITFELLGINL YELMKNNNFQ GFSLSIVRRF TLSVLKCLQM LSVEKIIHCD LKPENIVLYQ
241KGQASVKVID FGSSCYEHQK VYTYIQSRFY RSPEVILGHP YDVAIDMWSL GCITAELYTG
301YPLFPGENEV EQLACIMEVL GLPPAGFIQT ASRRQTFFDS KGFPKNITNN RGKKRYPDSK
361DLTMVLKTYD TSFLDFLRRC LVWEPSLRMT PDQALKHAWI HQSRNLKPQP RPQTLRKSNS
421FFPSETRKDK VQGCHHSSRK ADEITKETTE KTKDSPTKHV QHSGDQQDCL QHGADTVQLP
481QLVDAPKKSE AAVGAEVSMT SPGQSKNFSL KNTNVLPPIV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

520 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.