LSBio
DYRK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DYRK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DYRK3 antibodies →
$504.00each
Catalog Number: LS-G122532-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DYRK3 (NM_001004023), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DYRK3 | DYRK5 | RED | REDK | Protein kinase Dyrk3 | Regulatory erythroid kinase | HYAK3-2
Origin Species
Human
NCBI GenBank
NM_003582
SwissProt
O43781

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKWKEKLGDG VYDTFMMIDE TKCPPCSNVL CNPSEPPPPR RLNMTTEQFT GDHTQHFLDG
61GEMKVEQLFQ EFGNRKSNTI QSDGISDSEK CSPTVSQGKS SDCLNTVKSN SSSKAPKVVP
121LTPEQALKQY KHHLTAYEKL EIINYPEIYF VGPNAKKRHG VIGGPNNGGY DDADGAYIHV
181PRDHLAYRYE VLKIIGKGSF GQVARVYDHK LRQYVALKMV RNEKRFHRQA AEEIRILEHL
241KKQDKTGSMN VIHMLESFTF RNHVCMAFEL LSIDLYELIK KNKFQGFSVQ LVHKFAQSIL
301QSLDALHKNK IIHCDLKPEN ILLKHHGRSS TKVIDFGSSC FEYQKLYTYI QSRFYRAPEI
361ILGSRYSTPI DIWSFGCILA ELLTGQPLFP GEDEGDQLAC MMELLGMPPP KLLEQSKRAK
421YFINSKGIPR YCSVTTQADG RVVLVGGRSR RGKKRGPPGS KDWGTALKGC DDYLFIEFLK
481RCLHWDPSAR LTPAQALRHP WISKSVPRPL TTIDKVSGKR VVNPASAFQG LGSKLPPVVG
541IANKLKANLM SETNGSIPLC SVLPKLIS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

568 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.