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DYRK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DYRK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G117227-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DYRK2 (NM_003583), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DYRK2
Origin Species
Human
NCBI GenBank
NM_006482
SwissProt
Q92630

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNDHLHVGSH AHGQIQVQQL FEDNSNKRTV LTTQPNGLTT VGKTGLPVVP ERQLDSIHRR
61QGSSTSLKSM EGMGKVKATP MTPEQAMKQY MQKLTAFEHH EIFSYPEIYF LGLNAKKRQG
121MTGGPNNGGY DDDQGSYVQV PHDHVAYRYE VLKVIGKGSF GQVVKAYDHK VHQHVALKMV
181RNEKRFHRQA AEEIRILEHL RKQDKDNTMN VIHMLENFTF RNHICMTFEL LSMNLYELIK
241KNKFQGFSLP LVRKFAHSIL QCLDALHKNR IIHCDLKPEN ILLKQQGRSG IKVIDFGSSC
301YEHQRVYTYI QSRFYRAPEV ILGARYGMPI DMWSLGCILA ELLTGYPLLP GEDEGDQLAC
361MIELLGMPSQ KLLDASKRAK NFVSSKGYPR YCTVTTLSDG SVVLNGGRSR RGKLRGPPES
421REWGNALKGC DDPLFLDFLK QCLEWDPAVR MTPGQALRHP WLRRRLPKPP TGEKTSVKRI
481TESTGAITSI SKLPPPSSSA SKLRTNLAQM TDANGNIQQR TVLPKLVS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

528 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.