Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DYRK / DYRK1A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107674-100
Catalog Number: LS-G107674
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DYRK1A (NM_130436), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DYRK1 | DYRK | DYRK1A | HMNB | HP86 | MNB | MNB/DYRK protein kinase | MRD7 | MNBH | Serine/threonine kinase MNB | Yak1-related kinase
- Origin Species
- Human
- NCBI GenBank
- NM_001396
- SwissProt
- Q13627
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 84.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MHTGGETSAC KPSSVRLAPS FSFHAAGLQM AGQMPHSHQY SDRRQPNISD QQVSALSYSD61QIQQPLTNQR RMPQTFRDPA TAPLRKLSVD LIKTYKHINE VYYAKKKRRH QQGQGDDSSH121KKERKVYNDG YDDDNYDYIV KNGEKWMDRY EIDSLIGKGS FGQVVKAYDR VEQEWVAIKI181IKNKKAFLNQ AQIEVRLLEL MNKHDTEMKY YIVHLKRHFM FRNHLCLVFE MLSYNLYDLL241RNTNFRGVSL NLTRKFAQQM CTALLFLATP ELSIIHCDLK PENILLCNPK RSAIKIVDFG301SSCQLGQRIY QYIQSRFYRS PEVLLGMPYD LAIDMWSLGC ILVEMHTGEP LFSGANEVDQ361MNKIVEVLGI PPAHILDQAP KARKFFEKLP DGTWNLKKTK DGKREYKPPG TRKLHNILGV421ETGGPGGRRA GESGHTVADY LKFKDLILRM LDYDPKTRIQ PYYALQHSFF KKTADEGTNT481SNSVSTSPAM EQSQSSGTTS STSSSSGGSS GTSNSGRARS DPTHQHRHSG GHFTAAVQAM541DCETHSPQVR QQFPAPLGWS GTEAPTQVTV ETHPVQETTF HVAPQQNALH HHHGNSSHHH601HHHHHHHHHH GQQALGNRTR PRVYNSPTNS SSTQDSMEVG HSHHSMTSLS SSTTSSSTSS661SSTGNQGNQA YQNRPVAANT LDFGQNGAMD VNLTVYSNPR QETGIAGHPT YQFSANTGPA721HYMTEGHLTM RQGADREESP MTGVCVQQSP VASSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
754 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.