LSBio
DYNC1I1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DYNC1I1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G125460-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DYNC1I1 (NM_001135557), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNCI1 | DNCIC1 | DH IC-1 | DYNC1I1
Origin Species
Human
NCBI GenBank
NM_004411
SwissProt
O14576

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MSDKSDLKAE LERKKQRLAQ IREEKKRKEE ERKKKEADMQ QKKEPVQDDS DLDRKRRETE
61ALLQSIGISP EPPLVPTPMS PSSKSVSTPS EAGSQDSGDL GPLTRRRLHK LGVSKVTQVD
121FLPREVVSYS KETQTPLATH QSEEDEEDEE MVESKVGQDS ELENQDKKQE VKEAPPRELT
181EEEKQQILHS EEFLIFFDRT IRVIERALAE DSDIFFDYSG RELEEKDGDV QAGANLSFNR
241QFYDEHWSKH RVVTCMDWSL QYPELMVASY NNNEDAPHEP DGVALVWNMK FKKTTPEYVF
301HCQSSVMSVC FARFHPNLVV GGTYSGQIVL WDNRSHRRTP VQRTPLSAAA HTHPVYCVNV
361VGTQNAHNLI TVSTDGKMCS WSLDMLSTPQ ESMELVYNKS KPVAVTGMAF PTGDVNNFVV
421GSEEGTVYTA CRHGSKAGIG EVFEGHQGPV TGINCHMAVG PIDFSHLFVT SSFDWTVKLW
481TTKHNKPLYS FEDNADYVYD VMWSPVHPAL FACVDGMGRL DLWNLNNDTE VPTASVAIEG
541ASALNRVRWA QAGKEVAVGD SEGRIWVYDV GELAVPHNDE WTRFARTLVE IRANRADSEE
601EGTVELSA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

608 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.