LSBio
Dynactin 2 / Dynamitin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Dynactin 2 / Dynamitin Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DCTN2 antibodies →
$504.00each
Catalog Number: LS-G100804-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Dynamitin(DCTN2) (NM_006400), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DCTN50 | DYNAMITIN | DCTN-50 | Dynactin 2 (p50) | Dynactin complex 50 kD subunit | Dynactin subunit 2 | Dynactin 2 | Dynactin | 50 kd isoform | p50 dynamitin | RBP50 | DCTN2
Origin Species
Hu
NCBI GenBank
NM_006400
SwissProt
Q13561

Protein

Expression System
HEK293
Fusion Tag
FLAG, Myc
Molecular Weight
44.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MADPKYADLP GIARNEPDVY ETSDLPEDDQ AEFDAFAQEL EELTSTSVEH IIVNPNAAYD
61KFKDKRVGTK GLDFSDRIGK TKRTGYESGE YEMLGEGLGV KETPQQKYQR LLHEVQELTT
121EVEKIKTTVK ESATEEKLTP VLLAKQLAAL KQQLVASHLE KLLGPDAAIN LTDPDGALAK
181RLLLQLEATK NSKGGSGGKT TGTPPDSSLV TYELHSRPEQ DKFSQAAKVA ELEKRLTELE
241TAVRCDQDAQ NPLSAGLQGA CLMETVELLQ AKVSALDLAV LDQVEARLQS VLGKVNEIAK
301HKASVEDADT QSKVHQLYET IQRWSPIAST LPELVQRLVT IKQLHEQAMQ FGQLLTHLDT
361TQQMIANSLK DNTTLLTQVQ TTMRENLATV EGNFASIDER MKKLGK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

406 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.