Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DVL1 / DVL / Dishevelled Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104120-100
Catalog Number: LS-G104120
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DVL1(DVL1) (NM_182779), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DVL | DVL1L1 | DSH homolog 1 | Dishevelled | Dishevelled-1 | DVL1
- Origin Species
- Human
- NCBI GenBank
- NM_004421
- SwissProt
- O14640
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAETKIIYHM DEEETPYLVK LPVAPERVTL ADFKNVLSNR PVHAYKFFFK SMDQDFGVVK61EEIFDDNAKL PCFNGRVVSW LVLAEGAHSD AGSQGTDSHT DLPPPLERTG GIGDSRPPSF121HPNVASSRDG MDNETGTESM VSHRRERARR RNREEAARTN GHPRGDRRRD VGLPPDSAST181ALSSELESSS FVDSDEDGST SRLSSSTEQS TSSRLTRKYA SSLLKHGFLR HTVNKITFSE241QCYYVFGDLC SNLATLNLNS GSSGTSDQDT LAPLPHPAAP WPLGQGYPYQ YPGPPPCFPP301AYQDPGFSYG SGSTGSQQSE GSKSSGSTRS SRRAPGREKE RRAAGAGGSG SESDHTAPSG361VGSSWRERPA GQLSRGSSPR SQASATAPGL PPPHPTTKAY TVVGGPPGGP PVRELAAVPP421ELTGSRQSFQ KAMGNPCEFF VDIMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
444 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.