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DUSP8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DUSP8 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G116657-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DUSP8 (NM_004420), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C11orf81 | Dusp 8 | Dual specificity phosphatase 8 | DUSP8 | HB5 | HVH-5 | Hvh5 | HVH8 | M3/6 | VH5
Origin Species
Human
NCBI GenBank
NM_004420
SwissProt
Q13202

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGDRLPRKV MDAKKLASLL RGGPGGPLVI DSRSFVEYNS WHVLSSVNIC CSKLVKRRLQ
61QGKVTIAELI QPAARSQVEA TEPQDVVVYD QSTRDASVLA ADSFLSILLS KLDGCFDSVA
121ILTGGFATFS SCFPGLCEGK PAALLPMSLS QPCLPVPSVG LTRILPHLYL GSQKDVLNKD
181LMTQNGISYV LNASNSCPKP DFICESRFMR VPINDNYCEK LLPWLDKSIE FIDKAKLSSC
241QVIVHCLAGI SRSATIAIAY IMKTMGMSSD DAYRFVKDRR PSISPNFNFL GQLLEYERSL
301KLLAALQGDP GTPSGTPEPP PSPAAGAPLP RLPPPTSESA ATGNAAAREG GLSAGGEPPA
361PPTPPATSAL QQGLRGLHLS SDRLQDTNRL KRSFSLDIKS AYAPSRRPDG PGPPDPGEAP
421KLCKLDSPSG AALGLSSPSP DSPDAAPEAR PRPRRRPRPP AGSPARSPAH SLGLNFGDAA
481RQTPRHGLSA LSAPGLPGPG QPAGPGAWAP PLDSPGTPSP DGPWCFSPEG AQGAGGVLFA
541PFGRAGAPGP GGGSDLRRRE AARAEPRDAR TGWPEEPAPE TQFKRRSCQM EFEEGMVEGR
601ARGEELAALG KQASFSGSVE VIEVS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

625 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.