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DUS3L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DUS3L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111281-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DUS3L (NM_020175), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DUS3 | DUS3L
Origin Species
Human
NCBI GenBank
NM_001161619
SwissProt
Q96G46

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEGTAEAPL ENGGGGDSGA GALERGVAPI KRQYLTTKEQ FHQFLEAKGQ EKTCRETEVG
61DPAGNELAEP EAKRIRLEDG QTADGQTEEA AEPGEQLQTQ KRARGQNKGR PHVKPTNYDK
121NRLCPSLIQE SAAKCFFGDR CRFLHDVGRY LETKPADLGP RCVLFETFGR CPYGVTCRFA
181GAHLGPEGQN LVQEELAARG TQPPSIRNGL DKALQQQLRK REVRFERAEQ ALRRFSQGPT
241PAAAVPEGTA AEGAPRQENC GAQQVPAGPG TSTPPSSPVR TCGPLTDEDV VRLRPCEKKR
301LDIRGKLYLA PLTTCGNLPF RRICKRFGAD VTCGEMAVCT NLLQGQMSEW ALLKRHQCED
361IFGVQLEGAF PDTMTKCAEL LSRTVEVDFV DINVGCPIDL VYKKGGGCAL MNRSTKFQQI
421VRGMNQVLDV PLTVKIRTGV QERVNLAHRL LPELRDWGVA LVTLHGRSRE QRYTKLADWQ
481YIEECVQAAS PMPLFGNGDI LSFEDANRAM QTGVTGIMIA RGALLKPWLF TEIKEQRHWD
541ISSSERLDIL RDFTNYGLEH WGSDTQGVEK TRRFLLEWLS FLCRYVPVGL LERLPQRINE
601RPPYYLGRDY LETLMASQKA ADWIRISEML LGPVPPSFAF LPKHKANAYK
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

650 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.