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DTX3L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DTX3L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G102234-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DTX3L (NM_138287), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BBAP | Rhysin-2 | Rhysin2 | Deltex 3-like (Drosophila) | DTX3L | Protein deltex-3-like | Rhysin 2
Origin Species
Human
NCBI GenBank
NM_138287
SwissProt
Q8TDB6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASHLRPPSP LLVRVYKSGP RVRRKLESYF QSSKSSGGGE CTVSTQEHEA PGTFRVEFSE
61RAAKERVLKK GEHQILVDEK PVPIFLVPTE NSIKKNTRPQ ISSLTQSQAE TPSGDMHQHE
121GHIPNAVDSC LQKIFLTVTA DLNCNLFSKE QRAYITTLCP SIRKMEGHDG IEKVCGDFQD
181IERIHQFLSE QFLESEQKQQ FSPSMTERKP LSQQERDSCI SPSEPETKAE QKSNYFEVPL
241PYFEYFKYIC PDKINSIEKR FGVNIEIQES SPNMVCLDFT SSRSGDLEAA RESFASEFQK
301NTEPLKQECV SLADSKQANK FKQELNHQFT KLLIKEKGGE LTLLGTQDDI SAAKQKISEA
361FVKIPVKLFA ANYMMNVIEV DSAHYKLLET ELLQEISEIE KRYDICSKVS EKGQKTCILF
421ESKDRQVDLS VHAYASFIDA FQHASCQLMR EVLLLKSLGK ERKHLHQTKF ADDFRKRHPN
481VHFVLNQESM TLTGLPNHLA KAKQYVLKGG GMSSLAGKKL KEGHETPMDI DSDDSKAASP
541PLKGSVSSEA SELDKKEKGI CVICMDTISN KKVLPKCKHE FCAPCINKAM SYKPICPTCQ
601TSYGIQKGNQ PEGSMVFTVS RDSLPGYESF GTIVITYSMK AGIQTEEHPN PGKRYPGIQR
661TAYLPDNKEG RKVLKLLYRA FDQKLIFTVG YSRVLGVSDV ITWNDIHHKT SRFGGPEMYG
721YPDPSYLKRV KEELKAKGIE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

740 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.