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DTX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DTX2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G118819-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DTX2 (NM_001102596), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Deltex (Drosophila) homolog 2 | Deltex homolog 2 (Drosophila) | HDTX2 | KIAA1528 | Zinc ion binding protein | Protein deltex-2 | Deltex2 | DTX2 | RING finger protein 58 | RNF58
Origin Species
Human
NCBI GenBank
AB040961
SwissProt
Q86UW9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAMAPSPSLV QVYTSPAAVA VWEWQDGLGT WHPYSATVCS FIEQQFVQQK GQRFGLGSLA
61HSIPLGQADP SLAPYIIDLP SWTQFRQDTG TMRAVRRHLF PQHSAPGRGV VWEWLSDDGS
121WTAYEASVCD YLEQQVARGN QLVDLAPLGY NYTVNYTTHT QTNKTSSFCR SVRRQAGPPY
181PVTTIIAPPG HTGVACSCHQ CLSGSRTGPV SGRYRHSMTN LPAYPVPQHP PHRTASVFGT
241HQAFAPYNKP SLSGARSAPR LNTTNAWGAA PPSLGSQPLY RSSLSHLGPQ HLPPGSSTSG
301AVSASLPSGP SSSPGSVPAT VPMQMPKPSR VQQALAGATP KPEPEPEQVI KNYTEELKVP
361PDEDCIICME KLSTASGYSD VTDSKAIGSL AVGHLTKCSH AFHLLCLLAM YCNGNKDGSL
421QCPSCKTIYG EKTGTQPQGK MEVLRFQMSL PGHEDCGTIL IVYSIPHGIQ GPEHPNPGKP
481FTARGFPRQC YLPDNAQGRK VLELLKVAWK RRLIFTVGTS STTGETDTVV WNEIHHKTEM
541DRNITGHGYP DPNYLQNVLA ELAAQGVTED CLEQQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

575 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.