Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DTNA / Dystrobrevin Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108515-100
Catalog Number: LS-G108515
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Dystrobrevin alpha(DTNA) (NM_032979), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha-dystrobrevin | DTN-2 | DRP3 | DTN | DTN-3 | Dystrobrevin | alpha | DTN-1 | DTN-A | LVNC1 | D18S892E | DTNA | Dystrobrevin alpha | Dystrophin-related protein 3
- Origin Species
- Human
- NCBI GenBank
- NM_001391
- SwissProt
- Q9Y4J8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MIEDSGKRGN TMAERRQLFA EMRAQDLDRI RLSTYRTACK LRFVQKKCNL HLVDIWNVIE61ALRENALNNL DPNTELNVSR LEAVLSTIFY QLNKRMPTTH QIHVEQSISL LLNFLLAAFD121PEGHGKISVF AVKMALATLC GGKIMDKLRY IFSMISDSSG VMVYGRYDQF LREVLKLPTA181VFEGPSFGYT EQSARSCFSQ QKKVTLNGFL DTLMSDPPPQ CLVWLPLLHR LANVENVFHP241VECSYCHSES MMGFRYRCQQ CHNYQLCQDC FWRGHAGGSH SNQHQMKEYT SWKSPAKKLT301NALSKSLSCA SSREPLHPMF PDQPEKPLNL AHIVDTWPPR PVTSMNDTLF SHSVPSSGSP361FITRSMLESS NRLDEEHRLI ARYAARLAAE SSSSQPPQQR SAPDISFTID ANKQQRQLIA421ELENKNREIL QEIQRLRLEH EQASQPTPEK AQQNPTLLAE LRLLRQRKDE LEQRMSALQE481SRRELMVQLE GLMKLLKEEE LKQGVSYVPY CRSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
513 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.