Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DSTYK / RIPK5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101315-100
Catalog Number: LS-G101315
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RIPK5(DSTYK) (NM_015375), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Dusty PK | DustyPK | KIAA0472 | RIP-homologous kinase | RIPK5 | Sugen kinase 496 | RIP5 | DSTYK | Dusty protein kinase | HDCMD38P | Receptor interacting protein 5 | SgK496
- Origin Species
- Human
- NCBI GenBank
- AB007941
- SwissProt
- Q6XUX3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 105 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGDGVPWGS EPVSGPGPGG GGMIRELCRG FGRYRRYLGR LRQNLRETQK FFRDIKCSHN61HTCLSSLTGG GGAERGPAGD VAETGLQAGQ LSCISFPPKE EKYLQQIVDC LPCILILGQD121CNVKCQLLNL LLGVQVLPTT KLGSEESCKL RRLRFTYGTQ TRVSLALPGQ YELVHTLVAH181QGNWETIPEE DLEVQENNED AAHVLAELEV TMHHALLQEV DVVVAPCQGL RPTVDVLGDL241VNDFLPAITY ALHKDELSER DEQELQEIRK YFSFPVFFFK VPKLGSEIID SSTRRMESER301SPLYRQLIDL GYLSSSHWNC GAPGQDTKAQ SMLVEQSEKL RHLSTFSHQV LQTRLVDAAK361ALNLVHCHCL DIFINQAFDM QRDLQITPKR LEYTRKKENE LYESLMNIAN RKQEEMKDMI421VETLNTMKEE LLDDATNMEF KDVIVPENGE PVGTREIKCC IRQIQELIIS RLNQAVANKL481ISSVDYLRES FVGTLERCLQ SLEKSQDVSV HITSNYLKQI LNAAYHVEVT FHSGSSVTRM541LWEQIKQIIQ RITWVSPPAI TLEWKRKVAQ EAIESLSASK LAKSICSQFR TRLNSSHEAF601AASLRQLEAG HSGRLEKTED LWLRVRKDHA PRLARLSLES RSLQDVLLHR KPKLGQELGR661GQYGVVYLCD NWGGHFPCAL KSVVPPDEKH WNDLALEFHY MRSLPKHERL VDLHGSVIDY721NYGGGSSIAV LLIMERLHRD LYTGLKAGLT LETRLQIALD VVEGIRFLHS QGLVHRDIKL781KNVLLDKQNR AKITDLGFCK PEAMMSGSIV GTPIHMAPEL FTGKYDNSVD VYAFGILFWY841ICSGSVKLPE AFERCASKDH LWNNVRRGAR PERLPVFDEE CWQLMEACWD GDPLKRPLLG901IVQPMLQGIM NRLCKSNSEQ PNRGLDDSTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
929 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.