Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DSG4 / Desmoglein 4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104817-100
Catalog Number: LS-G104817
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Desmoglein 4(DSG4) (NM_177986), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cadherin family member 13 | CDGF13 | CDH family member 13 | Desmoglein 4 | Desmoglein-4 | LAH | CDHF13 | DSG4
- Origin Species
- Human
- SwissProt
- Q86SJ6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 111.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDWLFFRNIC LLIILMVVME VNSEFIVEVK EFDIENGTTK WQTVRRQKRE WIKFAAACRE61GEDNSKRNPI AKIRSDCESN QKITYRISGV GIDRPPYGVF TINPRTGEIN ITSVVDREIT121PLFLIYCRAL NSRGEDLERP LELRVKVMDI NDNAPVFSQS VYTASIEENS DANTLVVKLC181ATDADEENHL NSKIAYKIVS QEPSGAPMFI LNRYTGEVCT MSSFLDREQH SMYNLVVRGS241DRDGAADGLS SECDCRIKVL DVNDNFPTLE KTSYSASIEE NCLSSELIRL QAIDLDEEGT301DNWLAQYLIL SGNDGNWFDI QTDPQTNEGI LKVVKMLDYE QAPNIQLSIG VKNQADFHYS361VASQFQMHPT PVRIQVVDVR EGPAFHPSTM AFSVREGIKG SSLLNYVLGT YTAIDLDTGN421PATDVRYIIG HDAGSWLKID SRTGEIQFSR EFDKKSKYII NGIYTAEILA IDDGSGKTAT481GTICIEVPDI NDYCPNIFPE RRTICIDSPS VLISVNEHSY GSPFTFCVVD EPPGIADMWD541VRSTNATSAI LTAKQVLSPG FYEIPILVKD SYNRACELAQ MVQLYACDCD DNHMCLDSGA601AGIYTEDITG DTYGPVTEDQ AGVSNVGLGP AGIGMMVLGI LLLLLAPLLL LLCCCKQRQP661EGLGTRFAPV PEGGEGVMQS WRIEGAHPED RDVSNICAPM TASNTQDRMD SSEIYTNTYA721AGGTVEGGVS GVELNTGMGT AVGLMAAGAA GASGAARKRS STMGTLRDYA DADINMAFLD781SYFSEKAYAY ADEDEGRPAN DCLLIYDHEG VGSPVGSIGC CSWIVDDLDE SCMETLDPKF841RTLAEICLNT EIEPFPSHQA CIPISTDLPL LGPNYFVNES SGLTPSEVEF QEEMAASEPV901VHGDIIVTET YGNADPCVQP TTIIFDPQLA PNVVVTEAVM APVYDIQGNI CVPAELADYN961NVIYAERVLA SPGVPDMSNS STTEGCMGPV MSGNILVGPE IQVMQMMSPD LPIGQTVGST1021SPMTSRHRVT RYSNIHYTQQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1040 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.