Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DPYSL5 / CRMP5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111323-100
Catalog Number: LS-G111323
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CRMP5(DPYSL5) (NM_020134), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CRAM | CRMP3-associated molecule | DPYSL5 | DRP-5 | ULIP-6 | UNC33-like phosphoprotein 6 | CRMP-5 | CRMP5 | Dihydropyrimidinase-like 5 | Ulip6
- Origin Species
- Human
- NCBI GenBank
- NM_020134
- SwissProt
- Q9BPU6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLANSASVRI LIKGGKVVND DCTHEADVYI ENGIIQQVGR ELMIPGGAKV IDATGKLVIP61GGIDTSTHFH QTFMNATCVD DFYHGTKAAL VGGTTMIIGH VLPDKETSLV DAYEKCRGLA121DPKVCCDYAL HVGITWWAPK VKAEMETLVR EKGVNSFQMF MTYKDLYMLR DSELYQVLHA181CKDIGAIARV HAENGELVAE GAKEALDLGI TGPEGIEISR PEELEAEATH RVITIANRTH241CPIYLVNVSS ISAGDVIAAA KMQGKVVLAE TTTAHATLTG LHYYHQDWSH AAAYVTVPPL301RLDTNTSTYL MSLLANDTLN IVASDHRPFT TKQKAMGKED FTKIPHGVSG VQDRMSVIWE361RGVVGGKMDE NRFVAVTSSN AAKLLNLYPR KGRIIPGADA DVVVWDPEAT KTISASTQVQ421GGDFNLYENM RCHGVPLVTI SRGRVVYENG VFMCAEGTGK FCPLRSFPDT VYKKLVQREK481TLKVRGVDRT PYLGDVAVVV HPGKKEMGTP LADTPTRPVT RHGGMRDLHE SSFSLSGSQI541DDHVPKRASA RILAPPGGRS SGIWTRTRPL EQKLISEEDL AANDILDYKD DDDKV
595 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.