Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DPYSL3 / CRMP4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99426-100
Catalog Number: LS-G99426
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CRMP4(DPYSL3) (NM_001387), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CRMP4 | CRMP-4 | DRP3 | DRP-3 | LCRMP | ULIP1 | Unc-33-like phosphoprotein 1 | ULIP | ULIP-1 | Dihydropyrimidinase-like 3 | DPYSL3
- NCBI GenBank
- NM_001387
- SwissProt
- Q14195
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSYQGKKNIP RITSDRLLIK GGRIVNDDQS FYADIYMEDG LIKQIGDNLI VPGGVKTIEA61NGKMVIPGGI DVHTHFQMPY KGMTTVDDFF QGTKAALAGG TTMIIDHVVP EPESSLTEAY121EKWREWADGK SCCDYALHVD ITHWNDSVKQ EVQNLIKDKG VNSFMVYMAY KDLYQVSNTE181LYEIFTCLGE LGAIAQVHAE NGDIIAQEQT RMLEMGITGP EGHVLSRPEE LEAEAVFRAI241TIASQTNCPL YVTKVMSKSA ADLISQARKK GNVVFGEPIT ASLGIDGTHY WSKNWAKAAA301FVTSPPLSPD PTTPDYINSL LASGDLQLSG SAHCTFSTAQ KAIGKDNFTA IPEGTNGVEE361RMSVIWDKAV ATGKMDENQF VAVTSTNAAK IFNLYPRKGR ISVGSDSDLV IWDPDAVKIV421SAKNHQSAAE YNIFEGMELR GAPLVVICQG KIMLEDGNLH VTQGAGRFIP CSPFSDYVYK481RIKARRKMAD LHAVPRGMYD GPVFDLTTTP KGGTPAGSAR GSPTRPNPPV RNLHQSGFSL541SGTQVDEGVR SASKRIVAPP GGRSNITSLSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
570 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.