Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DPYSL2 / CRMP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G131086-100
Catalog Number: LS-G131086
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CRMP2(DPYSL2) (NM_001197293), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CRMP2 | CRMP-2 | DPYSL2 | DHPRP2 | Dihydropyrimidinase-like 2 | DRP-2 | N2A3 | ULIP2 | ULIP-2 | Unc-33-like phosphoprotein 2
- Origin Species
- Human
- NCBI GenBank
- NM_001386
- SwissProt
- Q16555
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAERKQSGKA AEDEEVPAFF KNLGSGSPKP RQKFCGMFCP VEGSSENKTI DFDSLSVGRG61SGQVVAQQRD VAHLGPDPQP PYSRQGRRAG GEPSVESGRK VEIRRASGKE ALQNINDQSD121RLLIKGGKIV NDDQSFYADI YMEDGLIKQI GENLIVPGGV KTIEAHSRMV IPGGIDVHTR181FQMPDQGMTS ADDFFQGTKA ALAGGTTMII DHVVPEPGTS LLAAFDQWRE WADSKSCCDY241SLHVDISEWH KGIQEEMEAL VKDHGVNSFL VYMAFKDRFQ LTDCQIYEVL SVIRDIGAIA301QVHAENGDII AEEQQRILDL GITGPEGHVL SRPEEVEAEA VNRAITIANQ TNCPLYITKV361MSKSSAEVIA QARKKGTVVY GEPITASLGT DGSHYWSKNW AKAAAFVTSP PLSPDPTTPD421FLNSLLSCGD LQVTGSAHCT FNTAQKAVGK DNFTLIPEGT NGTEERMSVI WDKAVVTGKM481DENQFVAVTS TNAAKVFNLY PRKGRIAVGS DADLVIWDPD SVKTISAKTH NSSLEYNIFE541GMECRGSPLV VISQGKIVLE DGTLHVTEGS GRYIPRKPFP DFVYKRIKAR SRLAELRGVP601RGLYDGPVCE VSVTPKTVTP ASSAKTSPAK QQAPPVRNLH QSGFSLSGAQ IDDNIPRRTT661QRIVAPPGGR ANITSLGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
677 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.