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DPY19L4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DPY19L4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DPY19L4 antibodies →
$504.00each
Catalog Number: LS-G104378-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DPY19L4 (NM_181787), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Dpy-19-like 4 (C. elegans) | Protein dpy-19 homolog 4 | Dpy-19-like protein 4 | DPY19L4
Origin Species
Human
NCBI GenBank
AA399598
SwissProt
Q7Z388

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEEEGPPVE LRQRKKPKSS ENKESAKEEK ISDIPIPERA PKHVLFQRFA KIFIGCLAAV
61TSGMMYALYL SAYHERKFWF SNRQELEREI TFQGDSAIYY SYYKDMLKAP SFERGVYELT
121HNNKTVSLKT INAVQQMSLY PELIASILYQ ATGSNEIIEP VYFYIGIVFG LQGIYVTALF
181VTSWLMSGTW LAGMLTVAWF VINRVDTTRI EYSIPLRENW ALPYFACQIA ALTGYLKSNL
241NTYGERFCYL LMSASTYTFM MMWEYSHYLL FLQAISLFLL DTFSVEQSDK VYEVYKIYIF
301SLFLGYLLQF ENPALLVSPL LSLVAALMLA KCLQLNVKKG SFVAKIIKVI NFYLVCTLTI
361TLNIIMKMFV PHKENGHMLK FLEVKFGLNM TKNFTMNWLL CQESLQAPSQ DFFLRLTQSS
421LLPFYILVLI ICFLSMLQVI FRRINGKSLK ETVTLEDGRI GERPEIIYHV IHTILLGSLA
481MVIEGLKYIW IPYVCMLAAF GVCSPELWMT LFKWLRLRTV HPILLALILS MAVPTIIGLS
541LWKEFFPRLM TELMELQEFY DPDTVELMTW IKRQAPVAAV FAGSPQLMGA IKLCTGWMVT
601SLPLYNDDDL LKRNENIYQI YSKRSAEDIY KILTSYKANY LIVEDAICNE VGPMRGCRVK
661DLLDIANGHM VCEEGDKLTY SKYGRFCHEV KINYSPYVNY FTRVYWNRSY FVYKINTVIS
721FQS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

723 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.