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DPY19L3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DPY19L3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DPY19L3 antibodies →
$725.00each
Catalog Number: LS-G102881-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DPY19L3 (NM_207325), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Dpy-19-like 3 (C. elegans) | Dpy-19-like protein 3 | DPY19L3 | Protein dpy-19 homolog 3
Origin Species
Human
SwissProt
Q6ZPD9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMSIRQRREI RATEVSEDFP AQEENVKLEN KLPSGCTSRR LWKILSLTIG GTIALCIGLL
61TSVYLATLHE NDLWFSNIKE VEREISFRTE CGLYYSYYKQ MLQAPTLVQG FHGLIYDNKT
121ESMKTINLLQ RMNIYQEVFL SILYRVLPIQ KYLEPVYFYI YTLFGLQAIY VTALYITSWL
181LSGTWLSGLL AAFWYVTNRI DTTRVEFTIP LRENWALPFF AIQIAAITYF LRPNLQPLSE
241RLTLLAIFIS TFLFSLTWQF NQFMMLMQAL VLFTLDSLDM LPAVKATWLY GIQITSLLLV
301CILQFFNSMI LGSLLISFNL SVFIARKLQK NLKTGSFLNR LGKLLLHLFM VLCLTLFLNN
361IIKKILNLKS DEHIFKFLKA KFGLGATRDF DANLYLCEEA FGLLPFNTFG RLSDTLLFYA
421YIFVLSITVI VAFVVAFHNL SDSTNQQSVG KMEKGTVDLK PETAYNLIHT ILFGFLALST
481MRMKYLWTSH MCVFASFGLC SPEIWELLLK SVHLYNPKRI CIMRYSVPIL ILLYLCYKFW
541PGMMDELSEL REFYDPDTVE LMNWINSNTP RKAVFAGSMQ LLAGVKLCTG RTLTNHPHYE
601DSSLRERTRA VYQIYAKRAP EEVHALLRSF GTDYVILEDS ICYERRHRRG CRLRDLLDIA
661NGHMMDGPGE NDPDLKPADH PRFCEEIKRN LPPYVAYFTR VFQNKTFHVY KLSRNK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

716 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.