Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DPS / PDSS1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114026-100
Catalog Number: LS-G114026
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DPS1(PDSS1) (NM_014317), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DPS | COQ10D2 | RP13-16H11.3 | SPS | TPRT | TPT 1 | Trans-prenyltransferase | PDSS1 | Trans-prenyltransferase (TPT) | Trans-prenyltransferase 1 | Coenzyme Q1 homolog | COQ1 | DPS1 | HDPS1 | TPT
- Origin Species
- Human
- NCBI GenBank
- NM_014317
- SwissProt
- Q5T2R2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASRWWRWRR GCSWKPAARS PGPGSPGRAG PLGPSAAAEV RAQVHRRKGL DLSQIPYINL61VKHLTSACPN VCRISRFHHT TPDSKTHSGE KYTDPFKLGW RDLKGLYEDI RKELLISTSE121LKEMSEYYFD GKGKAFRPII VALMARACNI HHNNSRHVQA SQRAIALIAE MIHTASLVHD181DVIDDASSRR GKHTVNKIWG EKKAVLAGDL ILSAASIALA RIGNTTVISI LTQVIEDLVR241GEFLQLGSKE NENERFAHYL EKTFKKTASL IANSCKAVSV LGCPDPVVHE IAYQYGKNVG301IAFQLIDDVL DFTSCSDQMG KPTSADLKLG LATGPVLFAC QQFPEMNAMI MRRFSLPGDV361DRARQYVLQS DGVQQTTYLA QQYCHEAIRE ISKLRPSPER DALIQLSEIV LTRDKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
415 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.