Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DPP2 / DPP7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101141-100
Catalog Number: LS-G101141
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DPP2(DPP7) (NM_013379), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Carboxytripeptidase | Dipeptidyl aminopeptidase II | Dipeptidyl peptidase 2 | DPPII | Dipeptidyl arylamidase II | Dipeptidyl-peptidase 7 | Dipeptidyl-peptidase II | Dipeptidylpeptidase 7 | Dipeptidyl peptidase 7 | Dipeptidyl peptidase II | DPP II | DPP2 | DPP7 | QPP
- Origin Species
- Human
- NCBI GenBank
- NM_013379
- SwissProt
- Q9UHL4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.33 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSAPWAPVL LLALGLRGLQ AGARRAPDPG FQERFFQQRL DHFNFERFGN KTFPQRFLVS61DRFWVRGEGP IFFYTGNEGD VWAFANNSGF VAELAAERGA LLVFAEHRYY GKSLPFGAQS121TQRGHTELLT VEQALADFAE LLRALRRDLG AQDAPAIAFG GSYGGMLSAY LRMKYPHLVA181GALAASAPVL AVAGLGDSNQ FFRDVTADFE GQSPKCTQGV REAFRQIKDL FLQGAYDTVR241WEFGTCQPLS DEKDLTQLFM FARNAFTVLA MMDYPYPTDF LGPLPANPVK VGCDRLLSEA301QRITGLRALA GLVYNASGSE HCYDIYRLYH SCADPTGCGT GPDARAWDYQ ACTEINLTFA361SNNVTDMFPD LPFTDELRQR YCLDTWGVWP RPDWLLTSFW GGDLRAASNI IFSNGNLDPW421AGGGIRRNLS ASVIAVTIQG GAHHLDLRAS HPEDPASVVE ARKLEATIIG EWVKAARREQ481QPALRGGPRL SLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
492 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.