Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DPF1 / Neuro-D4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125407-100
Catalog Number: LS-G125407
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Neuro D4(DPF1) (NM_001135155), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BAF45b | BRG1-associated factor 45B | DPF1 | NEUD4 | Neuro-d4 | Neuro-d4 homolog | Zinc finger protein neuro-d4
- Origin Species
- Human
- NCBI GenBank
- NM_004647
- SwissProt
- Q92782
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGGLSARPTA GRTDPAGTCW GQDPGSKMAT VIPGPLSLGE DFYREAIEHC RSYNARLCAE61RSLRLPFLDS QTGVAQNNCY IWMEKTHRGP GLAPGQIYTY PARCWRKKRR LNILEDPRLR121PCEYKIDCEA PLKKEGGLPE GPVLEALLCA ETGEKKIELK EEETIMDCQK QQLLEFPHDL181EVEDLEDDIP RRKNRAKGKA YGIGGLRKRQ DTASLEDRDK PYVCDICGKR YKNRPGLSYH241YTHTHLAEEE GEENAERHAL PFHRKNNHKQ FYKELAWVPE AQRKHTAKKA PDGTVIPNGY301CDFCLGGSKK TGCPEDLISC ADCGRSGHPS CLQFTVNMTA AVRTYRWQCI ECKSCSLCGT361SENDDQLLFC DDCDRGYHMY CLSPPMAEPP EGSWSCHLCL RHLKEKASAY ITLTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
414 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.