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DPEP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DPEP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G124890-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DPEP3 (NM_001129758), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Dipeptidase 3 | DPEP3 | Membrane-bound dipeptidase 3 | Metallopeptidase (family M19)
Origin Species
Human
NCBI GenBank
NM_022357
SwissProt
Q9H4B8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIRTPLSASA HRLLLPGSRG RPPRNMQPTG REGSRALSRR YLRRLLLLLL LLLLRQPVTR
61AETTPGAPRA LSTLGSPSLF TTPGVPSALT TPGLTTPGTP KTLDLRGRAQ ALMRSFPLVD
121GHNDLPQVLR QRYKNVLQDV NLRNFSHGQT SLDRLRDGLV GAQFWSASVS CQSQDQTAVR
181LALEQIDLIH RMCASYSELE LVTSAEGLNS SQKLACLIGV EGGHSLDSSL SVLRSFYVLG
241VRYLTLTFTC STPWAESSTK FRHHMYTNVS GLTSFGEKVV EELNRLGMMI DLSYASDTLI
301RRVLEVSQAP VIFSHSAARA VCDNLLNVPD DILQLLKNGG IVMVTLSMGV LQCNLLANVS
361TVADHFDHIR AVIGSEFIGI GGNYDGTGRF PQGLEDVSTY PVLIEELLSR SWSEEELQGV
421LRGNLLRVFR QVEKVREESR AQSPVEAEFP YGQLSTSCHS HLVPQNGHQA THLEVTKQPT
481NRVPWRSSNA SPYLVPGLVA AATIPTFTQW LC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

512 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.