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DOK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DOK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DOK3 antibodies →
$725.00each
Catalog Number: LS-G109726-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DOK3 (NM_024872), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Docking protein 3 | DOK3 | Dok-like protein | DOKL
Origin Species
Human
NCBI GenBank
NM_024872
SwissProt
Q7L591

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MTRGARLRSD ARAQLNQLSL DGGTGSGQKG KCEEFPSSLS SVSPGLEAAA LLLAVTMDPL
61ETPIKDGILY QQHVKFGKKC WRKVWALLYA GGPSGVARLE SWEVRDGGLG AAGDRSAGPG
121RRGERRVIRL ADCVSVLPAD GESCPRDTGA FLLTTTERSH LLAAQHRQAW MGPICQLAFP
181GTGEASSGST DAQSPKRGLV PMEENSIYSS WQEVGEFPVV VQRTEAATRC QLKGPALLVL
241GPDAIQLREA KGTQALYSWP YHFLRKLGSD KGVFSFEAGR RCHSGEGLFA FSTPCAPDLC
301RAVAGAIARQ RERLPELTRP QPCPLPRATS LPSLDTPGEL REMPPGPEPP TSRKMHLAEP
361GPQSLPLLLG PEPNDLASGL YASVCKRASG PPGNEHLYEN LCVLEASPTL HGGEPEPHEG
421PGSRSPTTSP IYHNGQDLSW PGPANDSTLE AQYRRLLELD QVEGTGRPDP QAGFKAKLVT
481LLSRERRKGP APCDRP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

496 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.