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DOCK8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DOCK8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DOCK8 antibodies →
$725.00each
Catalog Number: LS-G103074-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DOCK8 (NM_203447), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
SwissProt
Q8NF50

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
238.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTHLNSLDVQ LAQELGDFTD DDLDVVFTPK ECRTLQPSLP EEGVELDPHV RDCVQTYIRE
61WLIVNRKNQG SPEICGFKKT GSRKDFHKTL PKQTFESETL ECSEPAAQAG PRHLNVLCDV
121SGKGPVTACD FDLRSLQPDK RLENLLQQVS AEDFEKQNEE ARRTNRQAEL FALYPSVDEE
181DAVEIRPVPE CPKEHLGNRI LVKLLTLKFE IEIEPLFASI ALYDVKERKK ISENFHCDLN
241SDQFKGFLRA HTPSVAASSQ ARSAVFSVTY PSSDIYLVVK IEKVLQQGEI GDCAEPYTVI
301KESDGGKSKE KIEKLKLQAE SFCQRLGKYR MPFAWAPISL SSFFSVSTLE REVTDVDSVV
361GRSSVGERRT LAQSRRLSER ALSLEENGVG SNFKTSTLSV SIFFKQEGDR LSDEDLFKFL
421ADYKRSSSLQ RRVKSIPGLL RLEISTAPEI INCCLTPEML PVKPFPENRT RPHKEILEFP
481TREVYVPHTV YRNLLYVYPQ RLNFVNKLAS ARNITIKIQF MCGEDASNAM PVIFGKSSGP
541EFLQEVYTAV TYHNKSPDFY EEVKIKLPAK LTVNHHLLFT FYHISCQQKQ GASVETLLGY
601SWLPILLNER LQTGSYCLPV ALEKLPPNYS MHSAEKVPLQ NPPIKWAEGH KGVFNIEVQA
661VSSVHTQDNH LEKFFTLCHS LESQVTFPIR VLDQKISEMA LEHELKLSII CLNSSRLEPL
721VLFLHLVLDK LFQLSVQPMV IAGQTANFSQ FAFESVVAIA NSLHNSKDLS KDQHGRNCLL
781ASYVHYVFRL PEVQRDVPKS DAPTALLDPR SYHTYGRTSA AAVSSKLLQA RVMSSSNPDL
841AGTHSAADEE VKNIMSSKIA DRNCSRMSYY CSGSSDAPSS PAAPRPASKK HFHEELALQM
901VVSTGMVRET VFKYAWFFFE LLVKSMAQHV HNMDKRDSFR RTRFSDRFMD DITTIVNVVT
961SEIAALLVKP QKENEQAEKM NISLAFFLYD LLSLMDRGFV FNLIRHYCSQ LSAKLSNLPT
1021LISMRLEFLR ILCSHEHYLN LNLFFMNADT APTSPCPSIS SQNSSSCSSF QDQKIASMFD
1081LTSEYRQQHF LTGLLFTELA AALDAEGEGI SKVQRKAVSA IHSLLSSHDL DPRCVKPEVK
1141VKIAALYLPL VGIILDALPQ LCDFTVADTR RYRTSGSDEE QEGAGAINQN VALAIAGNNF
1201NLKTSGIVLS SLPYKQYNML NADTTRNLMI CFLWIMKNAD QSLIRKWIAD LPSTQLNRIL
1261DLLFICVLCF EYKGKQSSDK VSTQVLQKSR DVKARLEEAL LRGEGARGEM MRRRAPGNDR
1321FPGLNENLRW KKEQTHWRQA NEKLDKTKAE LDQEALISGN LATEAHLIIL DMQENIIQAS
1381SALDCKDSLL GGVLRVLVNS LNCDQSTTYL THCFATLRAL IAKFGDLLFE EEVEQCFDLC
1441HQVLHHCSSS MDVTRSQACA TLYLLMRFSF GATSNFARVK MQVTMSLASL VGRAPDFNEE
1501HLRRSLRTIL AYSEEDTAMQ MTPFPTQVEE LLCNLNSILY DTVKMREFQE DPEMLMDLMY
1561RIAKSYQASP DLRLTWLQNM AEKHTKKKCY TEAAMCLVHA AALVAEYLSM LEDHSYLPVG
1621SVSFQNISSN VLEESVVSED TLSPDEDGVC AGQYFTESGL VGLLEQAAEL FSTGGLYETV
1681NEVYKLVIPI LEAHREFRKL TLTHSKLQRA FDSIVNKDHK RMFGTYFRVG FFGSKFGDLD
1741EQEFVYKEPA ITKLPEISHR LEAFYGQCFG AEFVEVIKDS TPVDKTKLDP NKAYIQITFV
1801EPYFDEYEMK DRVTYFEKNF NLRRFMYTTP FTLEGRPRGE LHEQYRRNTV LTTMHAFPFI
1861KTRISVIQKE EFVLTPIEVA IEDMKKKTLQ LAVAINQEPP DAKMLQMVLQ GSVGATVNQG
1921PLEVAQVFLA EIPADPKLYR HHNKLRLCFK EFIMRCGEAV EKNKRLITAD QREYQQELKK
1981NYNKLKENLR PMIERKIPEL YKPIFRVESQ KRDSFHRSSF RKCETQLSQG S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2031 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.