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DNTTIP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DNTTIP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DNTTIP2 antibodies →
$725.00each
Catalog Number: LS-G113845-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TDIF2(DNTTIP2) (NM_014597), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNTTIP2 | Acidic 82 kDa protein mRNA | FCF2 | ERBP | HSU15552 | LPTS-interacting protein 2 | TdT-interacting factor 2 | TdIF2 | LPTS-RP2
Origin Species
Human
NCBI GenBank
NM_014597
SwissProt
Q5QJE6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVVTRSARAK ASIQAASAES SGQKSFAANG IQAHPESSTG SDARTTAESQ TTGKQSLIPR
61TPKARKRKSR TTGSLPKGTE PSTDGETSEA ESNYSVSEHH DTILRVTRRR QILIACSPVS
121SVRKKPKVTP TKESYTEEIV SEAESHVSGI SRIVLPTEKT TGARRSKAKS LTDPSQESHT
181EAISDAETSS SDISFSGIAT RRTRSMQRKL KAQTEKKDSK IVPGNEKQIV GTPVNSEDSD
241TRQTSHLQAR SLSEINKPNF YNNDFDDDFS HRSSENILTV HEQANVESLK ETKQNCKDLD
301EDANGITDEG KEINEKSSQL KNLSELQDTS LQQLVSQRHS TPQNKNAVSV HSNLNSEAVM
361KSLTQTFATV EVGRWNNNKK SPIKASDLTK FGDCGGSDDE EESTVISVSE DMNSEGNVDF
421ECDTKLYTSA PNTSQGKDNS VLLVLSSDES QQSENSENEE DTLCFVENSG QRESLSGDTG
481SLSCDNALFV IDTTPGMSAD KNFYLEEEDK ASEVAIEEEK EEEEDEKSEE DSSDHDENED
541EFSDEEDFLN STKAKLLKLT SSSIDPGLSI KQLGGLYINF NADKLQSNKR TLTQIKEKKK
601NELLQKAVIT PDFEKNHCVP PYSESKYQLQ KKRRKERQKT AGDGWFGMKA PEMTNELKND
661LKALKMRASM DPKRFYKKND RDGFPKYFQI GTIVDNPADF YHSRIPKKQR KRTIVEELLA
721DSEFRRYNRR KYSEIMAEKA ANAAGKKFRK KKKFRN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

756 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.