Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DNTTIP1 / TDIF1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102158-100
Catalog Number: LS-G102158
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNTTIP1 (NM_052951), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C20orf167 | DJ447F3.4 | Tdif1 | TdT-interacting factor 1 | TdT binding protein | DNTTIP1
- Origin Species
- Human
- NCBI GenBank
- NM_052951
- SwissProt
- Q9H147
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGATGDAEQP RGPSGAERGG LELGDAGAAG QLVLTNPWNI MIKHRQVQRR GRRSQMTTSF61TDPAISMDLL RAVLQPSINE EIQTVFNKYM KFFQKAALNV RDNVGEEVDA EQLIQEACRS121CLEQAKLLFS DGEKVIPRLT HELPGIKRGR QAEEECAHRG SPLPKKRKGR PPGHILSSDR181AAAGMVWKPK SCEPIRREGP KWDPARLNES TTFVLGSRAN KALGMGGTRG RIYIKHPHLF241KYAADPQDKH WLAEQHHMRA TGGKMAYLLI EEDIRDLAAS DDYRGCLDLK LEELKSFVLP301SWMVEKMRKY METLRTENEH RAVEAPPQTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
329 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.