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DNHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DNHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105232-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNHD1 (NM_173589), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MVPEERRVGL SSDETSSDSL KSWHSICVLD SKEQPLACQQ KQRQFVKPVT ESEQPTVLEL
61LLAELRTLFS AVLQDSSPAA WRYLHAVLGL LPPYRELLVG HLDLLPFLEQ LYCWAPWVQT
121HLHLDLLGAI VQAFPPDSSL LDSASHADCC PQKRRLHHRP PCPACPFVQA QWSRQQVKEE
181LATWLRPLTL PELQRCLGIV GAQVALEEAV WLDGLSLLPL ALAADIPVRY ESSDTDNAEV
241EPVGRKETRS QLDYEVPREK AFQKSSTGFS PETSFLDSQV MTALKMERYL KKIHFLYLNV
301APSRYFRPYS LMVVPPDKVN PEHYIFSPFG ILHVHPVEGS ETMTLGTWHH HCVLWQQLQF
361IPFFKYCLLR KSFTCWKKNV RLQGLHRLQK FLENHLLLAV PHFGAGLLHI SRLLQELHSV
421SWLPQELDRC YELLDLQTAL AEEKHKALRL LHRCLNLCTS ILRLVHEDTY HMQQCLQERV
481QNCDRIRTGQ GSIYLQRVQH KQLEQKLKQA EAWWLQLGKF ARLVDYMICQ SLISVLEEQI
541TSFVANILQA PRQKPFLSSQ LVFDDHGQLS HVPCVENMIQ TLTGGLQSVK TSALQVF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

597 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.