LSBio
DNASE2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DNASE2B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DNASE2B antibodies →
$504.00each
Catalog Number: LS-G110677-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNASE2B (NM_021233), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Deoxyribonuclease II beta | Deoxyribonuclease-2-beta | Endonuclease DLAD | DNase II beta | DLAD | DNase II-like acid DNase | DNase2-like acid DNase | DNASE2B | Lysosomal DNase II
Origin Species
Human
NCBI GenBank
NM_021233
SwissProt
Q8WZ79

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
38.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MMARLLRTSF ALLFLGLFGV LGAATISCRN EEGKAVDWFT FYKLPKKQNK ESGETGLEYL
61YLDSTTRSWR KSEQLMNDTK SVLGRTLQQL YEAYASKSNN TAYLIYNDGV PKPVNYSRKY
121GHTKGLLLWN RVQGFWLIHS IPQFPPIPEE GYDYPPTGRR NGQSGICITF KYNQYEAIDS
181QLLVCNPNVY SCSIPATFHQ ELIHMPQLCT RASSSEIPGR LLTTLQSAQG QKFLHFAKSD
241SFLDDIFAAW MAQRLKTHLL TETWQRKRQE LPSNCSLPYH VYNIKAIKLS RHSYFSSYQD
301HAKWCISQKG TKNRWTCIGD LNRSPHQAFR SGGFICTQNW QIYQAFQGLV LYYESCK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

357 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.