Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DNAJC22 / FLJ13236 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109694-100
Catalog Number: LS-G109694
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNAJC22 (NM_024902), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DNAJC22 | FLJ13236 | Wus | Wurst homolog
- Origin Species
- Human
- NCBI GenBank
- NM_024902
- SwissProt
- Q8N4W6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAKGLLVTYA LWAVGGPAGL HHLYLGRDSH ALLWMLTLGG GGLGWLWEFW KLPSFVAQAN61RAQGQRQSPR GVTPPLSPIR FAAQVIVGIY FGLVALISLS SMVNFYIVAL PLAVGLGVLL121VAAVGNQTSD FKNTLGSAFL TSPIFYGRPI AILPISVAAS ITAQRHRRYK ALVASEPLSV181RLYRLGLAYL AFTGPLAYSA LCNTAATLSY VAETFGSFLN WFSFFPLLGR LMEFVLLLPY241RIWRLLMGET GFNSSCFQEW AKLYEFVHSF QDEKRQLAYQ VLGLSEGATN EEIHRSYQEL301VKVWHPDHNL DQTEEAQRHF LEIQAAYEVL SQPRKPWGSR RTRTRPLEQKLISEEDLAANDILDYKDDDDKV
341 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.