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DNAJC14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DNAJC14 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108853-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNAJC14 (NM_032364), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNAJC14 | DRIP78 | DnaJ protein homolog 3 | Human DnaJ protein 3 | LIP6 | LYST-interacting protein LIP6 | DnaJ | HDj-3 | HDJ3
Origin Species
Human
NCBI GenBank
NM_032364
SwissProt
Q6Y2X3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAQKHPGERG LYGAHHSGGA SLRTLGPSVD PEIPSFSGLR DSAGTAPNGT RCLTEHSGPK
61HTQHPNPAHW LDPSHGPPGG PGPPRDAEDP DQSETSSEEE SGVDQELSKE NETGNQKDGN
121SFLSIPSACN CQGTPGIPEG PYSEGGNGSS SNFCHHCTSP ALGEDELEEE YDDEESLKFP
181SDFSRVSSGK KPPSRRQRHR FPTKEDTREG GRRDPRSPGR HRLGRKRSQA DKRKGLGLWG
241AEELCQLGQA GFWWLIELLV LVGEYVETCG HLIYACRQLK SSDLDLFRVW MGVWTGRLGG
301WAQVMFQFLS QGFYCGVGLF TRFLKLLGAL LLLALALFLG FLQLGWRFLV GLGDRLGWRD
361KATWLFSWLD SPALQRCLTL LRDSRPWQRL VRIVQWGWLE LPWVKQNINR QGNAPVASGR
421YCQPEEEVAR LLTMAGVPED ELNPFHVLGV EATASDVELK KAYRQLAVMV HPDKNHHPRA
481EEAFKVLRAA WDIVSNAEKR KEYEMKRMAE NELSRSVNEF LSKLQDDLKE AMNTMMCSRC
541QGKHRRFEMD REPKSARYCA ECNRLHPAEE GDFWAESSML GLKITYFALM DGKVYDITEW
601AGCQRVGISP DTHRVPYHIS FGSRIPGTRG RQRATPDAPP ADLQDFLSRI FQVPPGQMPN
661GNFFAAPQPA PGAAAASKPN STVPKGEAKP KRRKKVRRPF QR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

702 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.