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DNAJC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DNAJC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110377-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNAJC1 (NM_022365), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNAJL1 | DnaJ protein homolog MTJ1 | DNAJC1 | HTJ1 | DnaJ-like protein | ERdj1 | MTJ1
Origin Species
Human
NCBI GenBank
NM_022365
SwissProt
Q96KC8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTAPCSQPAQ LPGRRQLGLV PFPPPPPRTP LLWLLLLLLA AVAPARGWES GDLELFDLVE
61EVQLNFYQFL GVQQDASSAD IRKAYRKLSL TLHPDKNKDE NAETQFRQLV AIYEVLKDDE
121RRQRYDDILI NGLPDWRQPV FYYRRVRKMS NAELALLLFI ILTVGHYAVV WSIYLEKQLD
181ELLSRKKREK KKKTGSKSVD VSKLGASEKN ERLLMKPQWH DLLPCKLGIW FCLTLKALPH
241LIQDAGQFYA KYKETRLKEK EDALTRTELE TLQKQKKVKK PKPEFPVYTP LETTYIQSYD
301HGTSIEEIEE QMDDWLENRN RTQKKQAPEW TEEDLSQLTR SMVKFPGGTP GRWEKIAHEL
361GRSVTDVTTK AKQLKDSVTC SPGMVRLSEL KSTVQNSRPI KTATTLPDDM ITQREDAEGV
421AAEEEQEGDS GEQETGATDA RPRRRKPARL LEATAKPEPE EKSRAKRQKD FDIAEQNESS
481DEESLRKERA RSAEEPWTQN QQKLLELALQ QYPRGSSDRW DKIARCVPSK SKEDCIARYK
541LLVELVQKKK QAKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

554 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.