Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DNAJB1 / Hsp40 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100735-100
Catalog Number: LS-G100735
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Hsp40(DNAJB1) (NM_006145), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DnaJ protein homolog 1 | DNAJ1 | DNAJB1 | HDj-1 | Hdj1 | Heat shock 40 kDa protein 1 | Hsp40 | HSPF1 | RSPH16B | Sis1 | Radial spoke 16 homolog B | Heat shock 40kD protein 1 | Heat shock protein 40 | Human DnaJ protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_006145
- SwissProt
- P25685
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK61REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN121GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT181KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK241DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE301GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI TRTRPLEQKL ISEEDLAAND361ILDYKDDDDK V
371 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -80°C for long term storage.