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DNAH6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DNAH6 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105263-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNHL1 (NM_173645), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNAH6 | DNHL1 | Dynein heavy chain-like 1 | Dnahc6 | HL2 | KIAA1697 | HL-2 | Ciliary dynein heavy chain 6 | Dynein heavy chain 6 | axonemal
Origin Species
Human
NCBI GenBank
NM_001370
SwissProt
Q9C0G6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MTYTEQASKR HYCMRLTCFI RLNDYLIENT MHILTVNAVN SLLNHLTDKL KRTPSADVIQ
61KWITEEKPEV PDKKGTLMVE KQEEDESLIP MFLTELMLTV QSLLFEPSLE DFLDGILGAV
121NHCQNTVLSV PNLVPDSYFD AFTSPYINNK LEGKTCGTGP SLAAVFEDDK NFHTIISQIK
181ETIQAAFESA RIYAATFEKF QIFFKENESL DLQALKLQEP DINFFSEQLE KYHKQHKDAV
241ALRPTRNVGL LLIDTRLLRE KLIPSPLRCL EVLNFMLPRQ SKKKVDAIIF EAQDAEYKLE
301FVPTTTTEYV HSLLFLDEIQ ERIESLEDEG NIVTQMYKLM EQYQVPTPPE DFAVFATMKP
361SIVAVRNAID KSVGDRESSI KQFCVHLGSD LEELNNEVNE VKLQAQVS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

408 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.