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DNA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DNA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119324-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNA2 (NM_001080449), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNA2-like helicase | DNA2L | DNA2 | HDNA2 | KIAA0083
Origin Species
Human
NCBI GenBank
D42046
SwissProt
P51530

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
129.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKTPCIPSPK QGSKGRGQSP AAQVRGPTWN APAREGRPGA GHLGHLRVGA CARGAQARAF
61FPFLSFLCYP RFRCLFCLQF AIPASRMEQL NELELLMEKS FWEEAELPAE LFQKKVVASF
121PRTVLSTGMD NRYLVLAVNT VQNKEGNCEK RLVITASQSL ENKELCILRN DWCSVPVEPG
181DIIHLEGDCT SDTWIIDKDF GYLILYPDML ISGTSIASSI RCMRRAVLSE TFRSSDPATR
241QMLIGTVLHE VFQKAINNSF APEKLQELAF QTIQEIRHLK EMYRLNLSQD EIKQEVEDYL
301PSFCKWAGDF MHKNTSTDFP QMQLSLPSDN SKDNSTCNIE VVKPMDIEES IWSPRFGLKG
361KIDVTVGVKI HRGYKTKYKI MPLELKTGKE SNSIEHRSQV VLYTLLSQER RADPEAGLLL
421YLKTGQMYPV PANHLDKREL LKLRNQMAFS LFHRISKSAT RQKTQLASLP QIIEEEKTCK
481YCSQIGNCAL YSRAVEQQMD CSSVPIVMLP KIEEETQHLK QTHLEYFSLW CLMLTLESQS
541KDNKKNHQNI WLMPASEMEK SGSCIGNLIR MEHVKIVCDG QYLHNFQCKH GAIPVTNLMA
601GDRVIVSGEE RSLFALSRGY VKEINMTTVT CLLDRNLSVL PESTLFRLDQ EEKNCDIDTP
661LGNLSKLMEN TFVSKKLRDL IIDFREPQFI SYLSSVLPHD AKDTVACILK GLNKPQRQAM
721KKVLLSKDYT LIVGMPGTGK TTTICTLVRI LYACGFSVLL TSYTHSAVDN ILLKLAKFKI
781GFLRLGQIQK VHPAIQQFTE QEICRSKSIK SLALLEELYN SQLIVATTCM GINHPIFSRK
841IFDFCIVDEA SQISQPICLG PLFFSRRFVL VGDHQQLPPL VLNREARALG MSESLFKRLE
901QNKSAVVQLT VQYRMNSKIM SLSNKLTYEG KLECGSDKVA NAVINLRHFK DVKLELEFYA
961DYSDNPWLMG VFEPNNPVCF LNTDKVPAPE QVEKGGVSNV TEAKLIVFLT SIFVKAGCSP
1021SDIGIIAPYR QQLKIINDLL ARSIGMVEVN TVDKYQGRDK SIVLVSFVRS NKDGTVGELL
1081KDWRRLNVAI TRAKHKLILL GCVPSLNCYP PLEKLLNHLN SEKLIIDLPS REHESLCHIL
1141GDFQRE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1146 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.