LSBio
DMWD Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DMWD Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DMWD antibodies →
$725.00each
Catalog Number: LS-G116306-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human dystrophia myotonica containing WD repeat motif(DMWD) (NM_004943), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
D19S593E | DM9 | DMR-N9 | DMRN9 | DMWD | Protein DMR-N9 | Gene59 | Protein 59
Origin Species
Human
NCBI GenBank
L19267
SwissProt
Q09019

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAGGAEGGS GPGAAMGDCA EIKSQFRTRE GFYKLLPGDG AARRSGPASA QTPVPPQPPQ
61PPPGPASASG PGAAGPASSP PPAGPGPGPA LPAVRLSLVR LGEPDSAGAG EPPATPAGLG
121SGGDRVCFNL GRELYFYPGC CRRGSQRSID LNKPIDKRIY KGTQPTCHDF NQFTAATETI
181SLLVGFSAGQ VQYLDLIKKD TSKLFNEERL IDKTKVTYLK WLPESESLFL ASHASGHLYL
241YNVSHPCASA PPQYSLLKQG EGFSVYAAKS KAPRNPLAKW AVGEGPLNEF AFSPDGRHLA
301CVSQDGCLRV FHFDSMLLRG LMKSYFGGLL CVCWSPDGRY VVTGGEDDLV TVWSFTEGRV
361VARGHGHKSW VNAVAFDPYT TRAEEAATAA GADGERSGEE EEEEPEAAGT GSAGGAPLSP
421LPKAGSITYR FGSAGQDTQF CLWDLTEDVL YPHPPLARTR TLPGTPGTTP PAASSSRGGE
481PGPGPLPRSL SRSNSLPHPA GGGKAGGPGV AAEPGTPFSI GRFATLTLQE RRDRGAEKEH
541KRYHSLGNIS RGGSGGSGSG GEKPSGPVPR SRLDPAKVLG TALCPRIHEV PLLEPLVCKK
601IAQERLTVLL FLEDCIITAC QEGLICTWAR PGKAFTDEET EAQTGEGSWP RSPSKSVVEG
661ISSQPGNSPS GTVV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

674 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.