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DMRTA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DMRTA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DMRTA2 antibodies →
$725.00each
Catalog Number: LS-G109085-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DMRTA2 (NM_032110), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DMRT-like family A2 | DMRT5 | DMRTA2
Origin Species
Human
NCBI GenBank
AJ301580
SwissProt
Q96SC8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MELRSELPSV PGAATAAAAT ATGPPVASVA SVAAAAAAAA SLPVSVAGGL LRGPPLLLRA
61AEKYPRTPKC ARCRNHGVVS ALKGHKRYCR WKDCLCAKCT LIAERQRVMA AQVALRRQQA
121QEENEARELQ LLYGTAEGLA LAAANGIIPP RPAYEVFGSV CAADGGGPGA GAPAGTGGGA
181AGAGGSEAKL QKFDLFPKTL LQAGRPGSPL PPPVKPLSPD GADSGPGTSS PEVRPGSGSE
241NGDGESFSGS PLARASKEAG GSCPGSAGPG GGGEEDSPGS ASPLGSESGS EADKEEGEAA
301PAPGLGGGSG PRQRTPLDIL TRVFPGHRRG VLELVLQGCG GDVVQAIEQV LNHHRGGLAA
361GLGPAAPPDK AAVGAAAAAD DAWPSRVDAA AAAAAAAGGP GLPAPLQAGP AAPPHHRPLL
421AGAMAPGALG SLSSRSAFSP LQPNASHFGA DAGAYPLGAP LGLSPLRLAY SAAAAHSRGL
481AFMAPYSTAG LVPTLGFRPP MDYAFSDLMR DRSAAAAAAV HKEPTYGGGL YGPMVNGAPE
541KQ
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

542 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.