Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DMPK / DM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119754-100
Catalog Number: LS-G119754
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Dystrophia myotonica protein kinase(DMPK) (NM_001081562), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DM-kinase | DM1 | DM1PK | DMPK | DM protein kinase | DM1 protein kinase | DMK | MDPK | Myotonic dystrophy kinase | MT-PK | Myotonin kinase | MtPK | Myotonic protein kinase | Myotonin protein kinase A | Thymopoietin homolog | DM | Myotonin-protein kinase
- Origin Species
- Human
- NCBI GenBank
- NM_004409
- SwissProt
- Q09013
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 69.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSAEVRLRRL QQLVLDPGFL GLEPLLDLLL GVHQELGASE LAQDKYVADF LQWAEPIVVR61LKEVRLQRDD FEILKVIGRG AFSEVAVVKM KQTGQVYAMK IMNKWDMLKR GEVSCFREER121DVLVNGDRRW ITQLHFAFQD ENYLYLVMEY YVGGDLLTLL SKFGERIPAE MARFYLAEIV181MAIDSVHRLG YVHRDIKPDN ILLDRCGHIR LADFGSCLKL RADGTVRSLV AVGTPDYLSP241EILQAVGGGP GTGSYGPECD WWALGVFAYE MFYGQTPFYA DSTAETYGKI VHYKEHLSLP301LVDEGVPEEA RDFIQRLLCP PETRLGRGGA GDFRTHPFFF GLDWDGLRDS VPPFTPDFEG361ATDTCNFDLV EDGLTAMETL SDIREGAPLG VHLPFVGYSY SCMALRDSEV PGPTPMELEA421EQLLEPHVQA PSLEPSVSPQ DETAEVAVPA AVPAAEAEAE VTLRELQEAL EEEVLTRQSL481SREMEAIRTD NQNFASQLRE AEARNRDLEA HVRQLQERME LLQAEGATAV TGVPSPRATD541PPSHMAPRPW LWASARWWGQ APCTAATCCS LPGSLGLAYR RRFPCSCSPL FCLVPPPWAA601LGWWPTPANS PQSGAAQEPP ALPEPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
625 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.