Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DM4E3 / C11orf24 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110397-100
Catalog Number: LS-G110397
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C11orf24 (NM_022338), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C11orf24 | DM4E3 | Protein DM4E3
- Origin Species
- Human
- NCBI GenBank
- NM_022338
- SwissProt
- Q96F05
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWTALVLIWI FSLSLSESHA ASNDPRNFVP NKMWKGLVKR NASVETVDNK TSEDVTMAAA61SPVTLTKGTS AAHLNSMEVT TEDTSRTDVS EPATSGVAAD GVTSIAPTAV ASSTTAASIT121TAASSMTVAS SAPTTAASST TVASIAPTTA ASSMTAASST PMTLALPAPT STSTGRTPST181TATGHPSLST ALAQVPKSSA LPRTATLATL ATRAQTVATT ANTSSPMSTR PSPSKHMPSD241TAASPVPPMR PQAQGPISQV SVDQPVVNTT NKSTPMPSNT TPEPAPTPTV VTTTKAQARE301PTASPVPVPH TSPIPEMEAM SPTTQPSPMP YTQRAAGPGT SQAPEQVETE ATPGTDSTGP361TPRSSGGTKM PATDSCQPST QGQYMVVTTE PLTQAVVDKT LLLVVLLLGV TLFITVLVLF421ALQAYESYKK KDYTQVDYLI NGMYADSEMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
449 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.