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DLGAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DLGAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DLGAP2 antibodies →
$725.00each
Catalog Number: LS-G116440-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DLGAP2 (NM_004745), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DAP2 | DLGAP2 | DAP-2 | PSD-95/SAP90-binding protein 2 | SAPAP2
Origin Species
Human
NCBI GenBank
NM_004745
SwissProt
Q9P1A6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
108.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKGLSGSRTQ PPLCSGHTCG LAPPEDCEHL HHGPDARPPY LLSPADSCPG GRHRCSPRSS
61VHSECVMMPV VLGDHVSSST FPRMHYSSHY DTRDDCAVAH AGAKINRIPA NLLDQFEKQL
121PLHRDGFHTL QYQRTSAAAE QRSESPGRIR HLVHSVQKLF TKSHSLEGSS KSNANGTKAD
181GRADDHHHAH HAKHSKRSKS KERKPEGKPR PGMSSWWSSD DNLDSDSTYR TPSVLNRHHL
241GPVAHCYPDA LQSPFGDLSL KTSKSNNDVK CSACEGLALT PDAKYLKRSS WSTLTVSQAK
301EAYRKSSLNL DKPLLHQDAK PALRPCHYLQ VPQDEWGGYP TGGKDEEIPC RRMRSGSYIK
361AMGDEESGES DSSPKTSPKS AILPEPLLKS IGQRPLGEHQ TQTYLQAASD VPVGHSLDPA
421ANYNSPKFRS RNQSYMRAVS TLSQASCVSQ VSEAEINGQF ESVCESVFSE VESQAMDALD
481LPGCFRTRSH SYLRAIQAGY SQDDECIPMM TPSDITSTIR STAAVSYTNY KKTPPPVPPR
541TTSKPLISVT AQSSTESTQD AYQDSRAQRM SPWPQDSRGL YNSTDSLDSN KAMNLALETA
601AAQRHLPESQ SSSVRTSDKA ILVSKAEELL KSRCSSIGIQ DSEFPEHQPY PRSDVETATD
661SDTESRGLRE YHSVGVQVED EKRHGRFKRS NSVTAAVQAD LELEGFPGHI TTEDKGLQFG
721SSFQRHSEPS TPTQYSAVRT VRTQGLFSYR EDYRTQVDTS TLPPPDPWLE PAIDTVETGR
781MSPCRRDGSW FLKLLHAETK RMEGWCKEME REAEENDLSE EILGKIRSAV GSAQLLMSQK
841FQQFYWLCQQ NMDPSAMPRP TSQDLAGYWD MLQLSIEDVS MKFDELQRLR LNDWKMMESP
901ERKEERKVPP PIPKKPPKGK FPITREKSLD LPDRQRQEAR RRLMAAKRAA SFRQNSASER
961ADSIEIYIPE AQTRL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

975 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.