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DLGAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DLGAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G122505-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DLGAP1 (NM_001003809), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DAP-1 | DAP-1-ALPHA | DLGAP1 | HGKAP | PSD-95/SAP90 binding protein 1 | PSD-95/SAP90-binding protein 1 | SAPAP1 | DAP-1-BETA | DAP1 | GKAP
Origin Species
Human
NCBI GenBank
NM_004746
SwissProt
O14490

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNLIFHKDIL FGIPANKVPQ DEWTGYTPRG KDDEIPCRRM RSGSYIKAMG DEDSGDSDTS
61PKPSPKVAAR RESYLKATQP SLTELTTLKI SNEHSPKLQI RSHSYLRAVS EVSINRSLDS
121LDPAGLLTSP KFRSRNESYM RAMSTISQVS EMEVNGQFES VCESVFSELE SQAVEALDLP
181MPGCFRMRSH SYVRAIEKGC SQDDECVSLR SSSPPRTTTT VRTIQSSTVS SCITTYKKTP
241PPVPPRTTTK PFISITAQSS TESAQDAYMD GQGQRGDIIS QSGLSNSTES LDSMKALTAA
301IEAANAQIHG PASQHMGNNT ATVTTTTTIA TVTTEDRKKD HFKKNRCLSI GIQVDDAEEP
361DKTGENKAPS KFQSVGVQVE EEKCFRRFTR SNSVTTAVQA DLDFHDNLEN SLESIEDNSC
421PGPMARQFSR DASTSTVSIQ GSGNHYHACA ADDDFDTDFD PSILPPPDPW IDSITEDPLE
481AVQRSVCHRD GHWFLKLLQA ERDRMEGWCQ QMEREERENN LPEDILGKIR TAVGSAQLLM
541AQKFYQFREL CEENLNPNAH PRPTSQDLAG FWDMLQLSIE NISMKFDELH QLKANNWKQM
601DPLDKKERRA PPPVPKKPAK GPAPLIRERS LESSQRQEAR KRLMAAKRAA SVRQNSATES
661AESIEIYIPE AQTRL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

675 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.