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DLG5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DLG5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DLG5 antibodies →
$725.00each
Catalog Number: LS-G116442-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DLG5 (NM_004747), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Discs large protein LP-DLG | Discs large protein P-dlg | DLG5 | P-dlg | P-DLG5 | PDLG | KIAA0583 | Large type of P-DLG | LP-DLG | Disks large homolog 5 | Placenta and prostate DLG
Origin Species
Human
NCBI GenBank
NM_004747
SwissProt
Q8TDM6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
213.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPQRRELLA QCQQSLAQAM TEVEAVLGLL EAAGALSPGE RRQLDEEAGG AKAELLLKLL
61LAKERDHFQD LRAALEKTQP HLLPILYLNG VVGPPQPAEG AGSTYSVLST MPSDSESSSS
121LSSVGTTGKA PSPPPLLTDR QVNEKVENLS IQLRLMTRER NELRKRLAFA THGTAFDKRP
181YHRLNPDYER LKIQCVRAMS DLQSLQNQHT NALKRCEEVA KETDFYHTLH SRLLSDQTRL
241KDDVDMLRRE NGQLLRERNL LQQSWEDMKR LHEEDQKEIG DLRAQQQQVL KHNGSSEILN
301KLYDTAMDKL EVVKKDYDAL RKRYSEKVAI HNADLSRLEQ LGEENQRLLK QTEMLTQQRD
361TAIQLQHQCA LSLRRFEAIH HELNKATAQN KDLQWEMELL QSELTELRTT QVKTAKESEK
421YREERDAVYS EYKLIMSERD QVISELDKLQ TEVELAESKL KSSTSEKKAA NEEMEALRQI
481KDTVTMDAGR ANKEVEILRK QCKALCQELK EALQEADVAK CRRDWAFQER DKIVAERDSI
541RTLCDNLRRE RDRAVSELAE ALRSLDDTRK QKNDVSRELK ELKEQMESQL EKEARFRQLM
601AHSSHDSAID TDSMEWETEV VEFERETEDI DLKALGFDMA EGVNEPCFPG DCGIFVTKVD
661KGSIADGRLR VNDWLLRIND VDLINKDKKQ AIKALLNGEG AINMVVRRRK SLGGKVVTPL
721HINLSGQKDS GISLENGVYA AAVLPGSPAA KEGSLAVGDR IVAINGIALD NKSLNECESL
781LRSCQDSLTL SLLKVFPQSS SWSGQNIFEN IKDSDKMLSF RAHGPEVQAH NKRNLIQHNN
841STQTDIFYTD RLEDRKEPGP PGGSSSFLHK PFPGGPLQVC PQACPSASER SLSSFRSDAS
901GDRGFGLVDV RGRRPLLPFE TEVGPCGVGE ASLDKADSEG SNSGGTWPKA MLSSTAVPEK
961LSVYKKPKQR KSIFDPNTFK RPQTPPKIDY LLPGPGPAHS PQPSKRAGPL TPPKPPRRSD
1021SIKFQHRLET SSESEATLVG SSPSTSPPSA LPPDVDPGEP MHASPPRKAR VRIASSYYPE
1081GDGDSSHLPA KKSCDEDLTS QKVDELGQKR RRPKSAPSFR PKLAPVVIPA QFLEEQKCVP
1141ASGELSPELQ EWAPYSPGHS SRHSNPPLYP SRPSVGTVPR SLTPSTTVSS ILRNPIYTVR
1201SHRVGPCSSP PAARDAGPQG LHPSVQHQGR LSLDLSHRTC SDYSEMRATH GSNSLPSSAR
1261LGSSSNLQFK AERIKIPSTP RYPRSVVGSE RGSVSHSECS TPPQSPLNID TLSSCSQSQT
1321SASTLPRIAV NPASLGERRK DRPYVEEPRH VKVQKGSEPL GISIVSGEKG GIYVSKVTVG
1381SIAHQAGLEY GDQLLEFNGI NLRSATEQQA RLIIGQQCDT ITILAQYNPH VHQLSSHSRS
1441SSHLDPAGTH STLQGSGTTT PEHPSVIDPL MEQDEGPSTP PAKQSSSRIA GDANKKTLEP
1501RVVFIKKSQL ELGVHLCGGN LHGVFVAEVE DDSPAKGPDG LVPGDLILEY GSLDVRNKTV
1561EEVYVEMLKP RDGVRLKVQY RPEEFTKAKG LPGDSFYIRA LYDRLADVEQ ELSFKKDDIL
1621YVDDTLPQGT FGSWMAWQLD ENAQKIQRGQ IPSKYVMDQE FSRRLSMSEV KDDNSATKTL
1681SAAARRSFFR RKHKHKRSGS KDGKDLLALD AFSSDSIPLF EDSVSLAYQR VQKVDCTALR
1741PVLILGPLLD VVKEMLVNEA PGKFCRCPLE VMKASQQAIE RGVKDCLFVD YKRRSGHFDV
1801TTVASIKEIT EKNRHCLLDI APHAIERLHH MHIYPIVIFI HYKSAKHIKE QRDPIYLRDK
1861VTQRHSKEQF EAAQKLEQEY SRYFTGVIQG GALSSICTQI LAMVNQEQNK VLWIPACPL
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1919 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.