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DLC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DLC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DLC1 antibodies →
$725.00each
Catalog Number: LS-G100721-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DLC1 (NM_006094), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGAP7 | DLC1 | KIAA1723 | STARD12 | Deleted in liver cancer 1 | DLC-1 | HP protein | p122-RhoGAP
Origin Species
Human
NCBI GenBank
NM_182643

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
122.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCRKKPDTMI LTQIEAKEAC DWLRATGFPQ YAQLYEDFLF PIDISLVKRE HDFLDRDAIE
61ALCRRLNTLN KCAVMKLEIS PHRKRSDDSD EDEPCAISGK WTFQRDSKRW SRLEEFDVFS
121PKQDLVPGSP DDSHPKDGPS PGGTLMDLSE RQEVSSVRSL SSTGSLPSHA PPSEDAATPR
181TNSVISVCSS SNLAGNDDSF GSLPSPKELS SFSFSMKGHE KTAKSKTRSL LKRMESLKLK
241SSHHSKHKAP SKLGLIISGP ILQEGMDEEK LKQLNCVEIS ALNGNRINVP MVRKRSVSNS
301TQTSSSSSQS ETSSAVSTPS PVTRTRSLSA CNKRVGMYLE GFDPFNQSTF NNVMEQNFKN
361RESYPEDTVF YIPEDHKPGT FPKALTNGSF SPSGNNGSVN WRTGSFHGPG HISLRRENSS
421DSPKELKRRN SSSSMSSRLS IYDNVPGSIL YSSSGDLADL ENEDIFPELD DILYHVKGMQ
481RIVNQWSEKF SDEGDSDSAL DSVSPCPSSP KQIHLDVDND RTTPSDLDST GNSLNEPEEP
541SEIPERRDSG VGASLTRSNR HRLRWHSFQS SHRPSLNSVS LQINCQSVAQ MNLLQKYSLL
601KLTALLEKYT PSNKHGFSWA VPKFMKRIKV PDYKDRSVFG VPLTVNVQRT GQPLPQSIQQ
661AMRYLRNHCL DQVGLFRKSG VKSRIQALRQ MNEGAIDCVN YEGQSAYDVA DMLKQYFRDL
721PEPLMTNKLS ETFLQIYQYV PKDQRLQAIK AAIMLLPDEN REVLQTLLYF LSDVTAAVKE
781NQMTPTNLAV CLAPSLFHLN TLKRENSSPR VMQRKQSLGK PDQKDLNENL AATQGLAHMI
841AECKKLFQVP EEMSRCRNSY TEQELKPLTL EALGHLGNDD SADYQHFLQD CVDGLFKEVK
901EKFKGWVSYS TSEQAELSYK KVSEGPPLRL WRSVIEVPAV PEEILKRLLK EQHLWDVDLL
961DSKVIEILDS QTEIYQYVQN SMAPHPARDY VVLRTWRTNL PKGACALLLT SVDHDRAPVV
1021GVRVNVLLSR YLIEPCGPGK SKLTYMCRVD LRGHMPEWYT KSFGHLCAAE VVKIRDSFSN
1081QNTETKDTKS R
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1091 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.