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DIS3L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DIS3L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107545-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DIS3L (NM_133375), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DIS3-like exonuclease 1 | DIS3L1 | DIS3L | KIAA1955
Origin Species
Human
NCBI GenBank
NM_001143688
SwissProt
Q8TF46

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
110.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQTACQAVQH QRGRRQYNKL RNLLKDARHD CILFANEFQQ CCYLPRERGE SMEKWQTRSI
61YNAAVWYYHH CQDRMPIVMV TEDEEAIQQY GSETEGVFVI TFKNYLDNFW PDLKAAHELC
121DSILQSRRER ENESQESHGK EYPEHLPLEV LEAGIKSGRY IQGILNVNKH RAQIEAFVRL
181QGASSKDSDL VSDILIHGMK ARNRSIHGDV VVVELLPKNE WKGRTVALCE NDCDDKASGE
241SPSEPMPTGR VVGILQKNWR DYVVTFPSKE EVQSQGKNAQ KILVTPWDYR IPKIRISTQQ
301AETLQDFRVV VRIDSWESTS VYPNGHFVRV LGRIGDLEGE IATILVENSI SVIPFSEAQM
361CEMPVNTPES PWKVSPEEEQ KRKDLRKSHL VFSIDPKGCE DVDDTLSVRT LNNGNLELGV
421HIADVTHFVA PNSYIDIEAR TRATTYYLAD RRYDMLPSVL SADLCSLLGG VDRYAVSIMW
481ELDKASYEIK KVWYGRTIIR SAYKLFYEAA QELLDGNLSV VDDIPEFKDL DEKSRQAKLE
541ELVWAIGKLT DIARHVRAKR DGCGALELEG VEVCVQLDDK KNIHDLIPKQ PLEVHETVAE
601CMILANHWVA KKIWESFPHQ ALLRQHPPPH QEFFSELREC AKAKGFFIDT RSNKTLADSL
661DNANDPHDPI VNRLLRSMAT QAMSNALYFS TGSCAEEEFH HYGLALDKYT HFTSPIRRYS
721DIVVHRLLMA AISKDKKMEI KGNLFSNKDL EELCRHINNR NQAAQHSQKQ STELFQCMYF
781KDKDPATEER CISDGVIYSI RTNGVLLFIP RFGIKGAAYL KNKDGLVISC GPDSCSEWKP
841GSLQRFQNKI TSTTTDGESV TFHLFDHVTV RISIQASRCH SDTIRLEIIS NKPYKIPNTE
901LIHQSSPLLK SELVKEVTKS VEEAQLAQEV KVNIIQEEYQ EYRQTKGRSL YTLLEEIRDL
961ALLDVSNNYG ITRTRPLEQK LISEEDLAAN DILDYKDDDD KV

1002 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.