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Dipeptidase 2 / DPEP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Dipeptidase 2 / DPEP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110369-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DPEP2 (NM_022355), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DPEP2 | Dipeptidase 2
Origin Species
Human
NCBI GenBank
NM_022355
SwissProt
Q9H4A9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQPSGLEGPG TFGRWPLLSL LLLLLLLQPV TCAYTTPGPP RALTTLGAPR AHTMPGTYAP
61STTLSSPSTQ GLQEQARALM RDFPLVDGHN DLPLVLRQVY QKGLQDVNLR NFSYGQTSLD
121RLRDGLVGAQ FWSAYVPCQT QDRDALRLTL EQIDLIRRMC ASYSELELVT SAKALNDTQK
181LACLIGVEGG HSLDNSLSIL RTFYMLGVRY LTLTHTCNTP WAESSAKGVH SFYNNISGLT
241DFGEKVVAEM NRLGMMVDLS HVSDAVARRA LEVSQAPVIF SHSAARGVCN SARNVPDDIL
301QLLKKNGGVV MVSLSMGVIQ CNPSANVSTV ADHFDHIKAV IGSKFIGIGG DYDGAGKFPQ
361GLEDVSTYPV LIEELLSRGW SEEELQGVLR GNLLRVFRQV EKVQEENKWQ SPLEDKFPDE
421QLSSSCHSDL SRLRQRQSLT SGQELTEIPI HWTAKLPAKW SVSESSPHMA PVLAVVATFP
481VLILWL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

486 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.